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Cyclic AMP and the heat shock response in Chinese hamster ovary cells |
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| Authors: | S K Calderwood M A Stevenson G M Hahn |
| Affiliation: | 1. Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;3. ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Harwell Science and Innovation Campus, Didcot OX11 0QX, UK;4. PROTEOMASS Scientific Society, Madan Park, Rua dos Inventores, 2825-152 Caparica, Portugal;1. Department of Pathology, Immunology, and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ, USA |
| Abstract: | Heat shock leads to transient increases in cAMP levels in HA-1-CHO cells. Such pulses are correlated temporally with the induction of heat resistance (thermotolerance) and with heat shock protein synthesis. Although the kinetics of cAMP increase after heating suggest a role in thermotolerance induction, raising cAMP levels directly using dBcAMP did not produce full thermotolerance. The resistance induced by dBcAMP may thus be either a component of or different to heat-shock triggered resistance. Cells which had been made thermotolerant by heat shock did not produce a pulse in cAMP level on heating. The cAMP producing system thus seemed desensitized to heat in thermotolerant cells. |
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