Structural characterization of canine PYY |
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Authors: | V E Eysselein G A Eberlein D Grandt M Schaeffer B Zehres U Behn D Schaefer H Goebell M Davis T D Lee |
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Institution: | Harbor-UCLA Medical Center, Department of Gastroenterology, Torrance 90509. |
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Abstract: | PYY was purified from canine colonic mucosa by sequential steps of reverse phase HPLC and ion-exchange FPLC. Microsequence, amino acid and mass spectral analyses of the purified peptide and its tryptic fragments were consistent with the structure: YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-amide. Canine PYY(1-36) has the identical sequence as porcine and rat PYY but differs from human PYY at position 3, with Ala instead of Ile, and position 18, with Ser instead of Asn. A smaller form, PYY(3-36), was also purified and characterized. It may differ in its biological activity from the intact peptide and could act as a partial antagonist or agonist of PYY(1-36). |
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