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In Vitro Cultivation of the Vascular Phase of Sarcocystis capracanis and Sarcocystis tenella1
Authors:C. A. SPEER  R. J. CAWTHORN  J. P. DUBEY
Abstract:ABSTRACT. Sporozoites of Sarcocystis capracanis and S. tenella (Apicomplexa) penetrated all four cell types tested (bovine monocytes, BM; bovine pulmonary artery endothelial cells, CPA; Madin-Darby bovine kidney; and ovine monocytes). Sporozoites of S. tenella developed to meronts in BM and CPA; those of S. capracanis developed to meronts in BM only. Both species of Sarcocystis developed to large first-generation meronts followed by small meronts. At 40 to 50 days after inoculation (DAI) of sporozoites, considerably more merozoites of S. tenella were harvested from CPA (24.9 × 106 merozoites/75-cm2 flask; n = 4) than from BM (1.9 × 106 merozoites/75-cm2 flask; n = 4). Merozoites of S. capracanis were most numerous in BM at 88 to 100 DAI during which time 2.1 × 106 merozoites/75-cm2 flask (n = 4) were harvested.
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