Russian Article pp. 207–211

During the submerged cultivation of Trichoderma sp. 414 on a medium with 2% cellulose containing plant substrats an enzyme system – exocellobiohydrolase (C₁-enzyme), endogluconase (C_x-enzyme), β-glucosidase and xylase – catalizing the cellulose hydrolysis was synthesized. The process of enzyme biosynthesis by the microbial strain under the conditions of two-step cultivation in flasks was optimized. The influence of different sources of carbohydrate – avicel, micricel, maize stalk and straw – on the activity of the synthesized enzymes was studied. This activity depends on induction properties and the concentration of the used substrats. During the cultivation of Trichoderma sp. 414 on a medium containing avicel and wheat bran the activity of cellobiohydrolase reaches 40 U/ml and this of endogluconase – 520 U/ml. When the cultivation was performed on a medium containing wheat bran and straw the activity of xylanase reaches 240 U/ml.