干旱胁迫下发菜光合作用相关基因差异表达分析

该研究以不同失水处理的发菜为研究材料，以充分吸水状态的发菜为对照，利用高通量测序技术和qRT PCR技术检测了干旱胁迫下发菜光合作用相关基因差异表达规律，并对光合色素和酶活在干旱胁迫下的变化进行了研究。结果表明：(1)发菜在不同程度干旱胁迫下有113个光合作用相关基因差异表达，其中失水30%、75%和100%的发菜分别有44个、74个和91个光合作用相关基因差异表达。(2)随着干旱胁迫程度的加深藻胆素、叶绿素a和类胡萝卜素含量逐渐降低，Rubisco活性随着干旱胁迫程度的增强先上升后下降，GAPDH活性随着干旱胁迫的增强呈现下降的趋势。研究表明，发菜通过光合作用相关基因的差异表达调控光合作用以适应干旱胁迫。该研究结果对进一步研究发菜干旱胁迫响应机制及其耐旱光合机理奠定了基础。

Analysis of Differentially Expressed Genes Related to Photosynthesis in Nostoc flagelliforme under Drought Stress

In this study, the colonies of N. flagelliforme with different water loss treatments were used as the research material, and fully water absorbent state was used as a control. High throughput sequencing and qRT PCR techniques were used to detect the differential expression of photosynthesis related genes under drought stress, and the changes of photosynthetic pigments were also detected. The results showed that: (1) 113 photosynthesis related genes were differentially expressed under different degrees of drought stress, and 44, 74 and 91 photosynthesis related genes were differentially expressed in 30%, 75% and 100% dehydrated colonies, respectively. (2) The contents of phycobilin, chlorophyll and carotenoid decreased gradually with the drought intensifies, the Rubisco activity increased at first and then decreased but the activity of GAPDH decreased with the increase of drought stress. The results showed that N. flagelliforme regulates photosynthetic activity through the differential expression of photosynthesis related genes to adapt to drought stress. The research provides reference value for further research on the drought stress response mechanism of N. flagelliforme and lays a foundation for further understanding of the photosynthetic mechanism of N. flagelliforme drought tolerance.