The NADPH oxidase complex of phagocytic leukocytes: a biochemical and cytochemical view |
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| Authors: | John M. Robinson John A. Badwey |
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| Affiliation: | (1) Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, 333 W. 10th Avenue, 43210 Columbus, OH, USA;(2) Boston Biomedical Research Institute, 20 Staniford Street, 02114 Boston, MA, USA |
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| Abstract: | The NADPH oxidase complex catalyzes the formation of superoxide (O2–) in phagocytic leukocytes. This paper reviews recent advances in our understanding of this enzyme system. Recent studies have defined conditions for reconstitution of this enzymatic activity with purified proteins in a cell-free system. The role of the individual proteins that make up the active complex, their regulation and the effects of mutations in these proteins are discussed. While these studies represent major achievements, it is clear from cytochemical investigations that additional levels of complexity exist in the modulation of the NADPH oxidase complex in vivo. A major role for cytochemical analysis in understanding the cell biological aspects of the generation of reactive oxygen species is discussed.Portions of this review were presented at the 36th Symposium of the Society for Histochemistry, 21 September 1994, Heidelberg, Germany |
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