Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro |
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Authors: | E Duhoux B Sougoufara Y Dommergues |
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Institution: | (1) Départment de Biologie Végétale, Faculté des Sciences, Dakar, Sénégal;(2) Laboratoire de Microbiologie des sols, ORSTOM, Dakar, Sénégal;(3) BSSFT (CTFT/ORSTOM/CNRS), 45 bis Avenue de la Belle Gabrielle, F-94736 Nogent-sur-Marne Cedex, France |
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Abstract: | The study of the actinorhizal symbiosis in Casuarina equisetifolia requires an homogenous plant material. Consequently, we devised a method of micropropagation based on the use of immature female inflorescences (IFI) as explants. IFI excised from an adult tree formed multiple buds after 4-week incubation on Murashige and Skoog medium with 0.05 mol 1–1 NAA and 11.1 mol 1–1 BAP. The axillary buds evolved into 5–6 cm long shoots 5 weeks after the transfer of IFI on a similar medium except for the addition of activated charcoal. Rooting of the shoots was obtained on a third medium, without BAP or charcoal, but with 1 mol 1–1 NAA. The plantlets were transferred into soil. Their growth was satisfactory and no plagiotropic tendency was observed.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
3-indoleacetic acid
- IFI
immature female inflorescences
- NAA
-naphtaleneacetic acid |
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