Effect of ATP concentration and temperature on firefly luciferase activity |
| |
| Authors: | I. Gális J. Jirásková |
| |
| Affiliation: | 1. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Brani?ovská 31, 370 05, ?eské Budějovice, Czech Republic
|
| |
| Abstract: | The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
