Restriction fragment patterns of chloroplast and mitochondrial DNA of Dasypyvum villosum (L.) candargy and wheats |
| |
Authors: | G. Shu S. Muthukrishnan G. H. Liang G. M. Paulsen |
| |
Affiliation: | (1) Genetics Program, Kansas State University, 66506-5501 Manhattan, KS, USA;(2) Department of Biochemistry, Kansas State University, 66506-5501 Manhattan, KS, USA;(3) Department of Agronomy, Kansas State University, 66506-5501 Manhattan, KS, USA |
| |
Abstract: | To elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L.) candargy were compared with those of tetraploid wheat (Triticum durum Desf., PI265007), hexaploid wheat (Triticum aestivum L., cv Chinese Spring), Aegilops longissimum (S. and M., in Muschli) Bowden and Hordeum vulgare L. T. aestivum and T. durum had identical restriction patterns for their cp and mtDNAs in digestions with four different enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were distinct differences in chloroplast and mitochondrial DNA restriction fragment patterns between D. villosum and tetraploid and hexaploid wheats. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Himalaya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlikely to be the donor of cytoplasm to wheats, and its cytoplasmic organelles were also different from those of A. longissimum.Contribution No. 92-522-J from the Kansas Agricultural Experiment Station; Kansas State University, Manhattan, Kansas, USA |
| |
Keywords: | Triticum aestivum T. durum Aegilops longissimum Dasypyrum villosum Endonuclease Cytoplasm donor |
本文献已被 SpringerLink 等数据库收录! |
|