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Antioxidant and antimicrobial properties of ethanolic extract fromLepista nuda (Bull.) Cooke
Authors:Nazime Mercan  Mehmet Emin Duru  Ariz Turkoglu  Kudret Gezer  Ibrahim Kivrak  Hüseyin Turkoglu
Affiliation:1. Department of Biology, Faculty of Science and Arts, Pamukkale University, 20017, Denizli, Turkey
2. Department of Chemistry, Faculty of Science and Arts, Mugla University, 48000, Mugla, Turkey
3. Department of Science Educations, Faculty of Education, Pamukkale University, 20020, Denizli, Turkey
4. Department of Food Enginering, Faculty of Agriculture, Harran University, 63040, Sanliurfa, Turkey
Abstract:Antioxidant capacity and antimicrobial activities ofLepista nuda (Bull.) Cooke extracts obtained with ethanol were investigated. Four complementary test systems, namely DPPH free radical scavenging, β-carotene/linoleic acid systems, total phenolic compounds and total flavonoid concentration, have been used. Linoleic, acid inhibition values ofL. nuda ethanolic extract, BHA and α-to copherol standards were found to be 84.3% 98.9% and 99.2% respectively in the concentration of 160μg/ml. Total flavonoid amount was 8.21 ± 0.56 μg mg?1 quercetin equivalent while the phenolic compound amount was 48.01 ± 0.29 μg mg?1 pyrocatechol equivalent in the extract. The antimicrobial activity ofL. nuda extract was testedin vitro by using the agar-well diffusion method. TheL. nuda extract showed antibacterial activity againstMicrococcus flavus, Micrococcus luteus, Bacillus cereus, Yersinia enterocolitica, Staphylococcus aureus, Salmonella enteritidis andEscherichia coli. TheL. nuda extract did not exhibit antican didal activity againstCandida albicans. The extracts could be suitable as antimicrobial and antioxidativeagents in the food industry.
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