Cloning and sequence analysis ofglnB-like gene from nitrogen-fixingPaenibacillus polymyxa G2 |
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Authors: | Yanqin Ding Sanfeng Chen |
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Affiliation: | 1. National Key Laboratory for Agrobiotechnology and Biological College, China Agricultural University, 100094, Beijing, China 2. Department of Microbiology, Shandong Agricultural University, 271018, Shandong, China
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Abstract: | A 240 bpglnB-like gene fragment was PCR amplified fromPaenibacillus polymyxa G2 using universal degenerate primers. These degenerate primers, based on all knownglnB-like genes including thenrgB ofBacillus subtilis at the time of design, were chosen from highly conserved amino acid sequences. The GlnB-like sequence ofP. polymyxa G2 had relatively high identities (more than 70%) to other bacteria GlnB, e.g.Escherichia coli (78%) andKlebsiella pneuomoniae (79%). However, the identity of theP. polymyxa GlnB-like sequence to a GlnK homologue (NrgB) of B.subtilis was low (46%). This is the first report of the sequence of theglnB-like gene from the genusPaenibacillus. Knowledge of theglnB-like gene sequence ofP. polymyxa will make us study deeply the function ofglnB in the genusPaenibacillus. |
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