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Localisation of concanavalin A binding sites on the stigma surface of a grass species
Affiliation:1. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China;2. University of Chinese Academy of Sciences, No. 19 A Yuquan Road, Beijing 100049, China;3. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;4. College of Food, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China;5. Waters Technologies (Shanghai) Ltd., No. 1000 Jinhai Road, Shanghai 201203, China;1. State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering, Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, 730000, People''s Republic of China;2. Gansu Academy of Agricultural Sciences, Lanzhou, 730070, People''s Republic of China;3. Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People''s Republic of China;4. Department of Horticulture, Foshan University, Foshan, 528000, PR China;5. School of Land and Food, University of Tasmania, Private Bag 54, Hobart, Tasmania, 7001, Australia;1. Discipline of Geography, School of Agriculture, Earth and Environmental Science, University of KwaZulu-Natal, Pietermaritzburg, South Africa;2. Discipline of Anthropology, School of Social Science, University of KwaZulu-Natal, Durban, South Africa;1. Department of Fundamental and Theoretical Physics and Department of Nuclear Physics and Accelerator Sciences, Research School of Physics, The Australian National University, Canberra ACT 2601, Australia;2. Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37235, USA;3. Facility for Rare Isotope Beams, Michigan State University, East Lansing, MI 48824, USA
Abstract:The receptive surfaces of the stigma papillae of Phalaris minor (Gramineae) bear a superficial proteinaceous layer outside of the cuticle. Using the peroxidase labelling technique, it has been shown that this surface layer or pellicle binds the lectin concanavalin A. Glycoproteins in the pellicle may play a part in pollen adhesion as well as in pollen-stigma recognition.
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