Morphological effects and metabolism of the molting hormone in Aedes aegypti cultured cells |
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| Affiliation: | 1. School of Health and Medicine, Catholic University of Brasilia, Brasília, DF, Brazil;2. Department of Sports Methods and Techniques, Federal University of Santa Maria, Santa Maria, Brazil;3. Physical Education School, Gurupi University, Gurupi, Brazil;4. Division of Psychology and Mental Health, Faculty of Biology, Medicine and Health, University of Manchester, United Kingdom;5. NICM Health Research Institute, Western Sydney University, Westmead, Australia;6. Cambridge Centre for Sport and Exercise Sciences, Anglia Ruskin University, Cambridge, United Kingdom;7. Physiotherapy Department, South London and Maudsley NHS Foundation Trust, Denmark Hill, London, United Kingdom;8. Department of Psychological Medicine, Institute of Psychiatry, Psychology and Neuroscience, King''s College London, London, United Kingdom;9. INISA, Federal University of Mato Grosso do Sul, Campo Grande, Brazil |
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| Abstract: | Growth of Aedes aegypti cultured cells was arrested by α- and β-ecdysone at concentrations of 0.01 to 10.0 μg/ml. The inhibitory effect was accompanied by increased cell volume. Prolonged exposure of at least 24 hr to the molting hormones was necessary to induce the above effects. 3H-α-ecdysone was incorporated into the mosquito cultured cells, and 2.1% of the total label added could be detected in thoroughly washed cells. Thin-layer chromatography of the cellular butanolic extract revealed one apolar peak only and no traces of the original labeled α-ecdysone or its immediate hydroxylation product, e.g. β-ecdysone. It is suggested that the hormone was rapidly converted to a metabolite which did not correspond with the apolar 3-α-dehydro-ecdysone, as was initially speculated. |
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