Selective inhibition by clofenapate of glycerolipid synthesis via the esterification of dihydroxyacetone phosphate in rat liver slices |
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Affiliation: | Department of Biochemistry, University Hospital and Medical School, Clifton Boulevard, Nottingham NG7 2UH, England |
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Abstract: | - 1.1. The route of glycerol incorporation into glycerolipids was measured by incubating rat liver slices with [l-14C]-glycerol and (2-3H]-glycerol.
- 2.2. Approximately 75% of the incorporation was via the esterification of dihydroxyacetone phosphate with the remainder passing through the glycerol phosphate pathway.
- 3.3. Clofenapate (1.25mM) inhibited lipid synthesis by about 78% and this resulted from a selective inhibition of dihydroxyacetone phosphate incorporation.
- 4.4. A combined inhibition of glycerol phosphate oxidase and dihydroxyacetone phosphate acyltransferase is thought to be responsible for these observations.
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