Hormonal control of diacyl glycerol mobilization from fat body of the desert locust,Schistocerca gregaria |
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| Affiliation: | 1. Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA;2. Research Institute for Biotechnology and Environment, Nong Lam University, Ho Chi Minh City, Viet Nam;3. Department of Family and Preventive Medicine, University of Utah, School of Medicine, Salt Lake City, UT, USA;1. Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia;2. Western Sydney University, Advanced Materials Characterisation Facility, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia;3. Western Sydney University, NICM Health Research Institute, School of Science, Campbelltown Campus, Locked Bag 1797, Penrith, NSW 2751, Australia;4. South West Sydney Clinical Campuses, Discipline of Medicine, University of New South Wales Sydney, NSW 2170, Australia;5. Liverpool John Moores University, School of Pharmacy & Biomolecular Sciences, Liverpool L3 3AF, United Kingdom;6. Sorbonne University, Parisian Institute of Molecular Chemistry, UMR 8232, Polymer Chemistry team, 75252 Paris, France |
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| Abstract: | The action of adipokinetic hormone in stimulating mobilization of diacyl glycerol from locust fat body was studied in vitro. For optimum lipid mobilization haemolymph protein and calcium ions were required, and the amount and time course of release were dependent on the hormone concentration. Dibutyryl cyclic AMP or theophylline mimicked the action of the hormone and a synergistic effect of theophylline with hormone was found. Purified adipokinetic hormone with theophylline increased the concentration of cyclic AMP in fat body and stimulated the incorporation of [3H]-adenine into cyclic AMP.A lipase was present in fat body extracts which hydrolysed triacyl glycerol mainly to diacyl glycerol plus fatty acid. The activity of this enzyme was low, and attempts to activate it with hormone were unsuccessful. |
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