Mutagenic effect of sodium nitrite on cultured mouse cells |
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| Affiliation: | 1. 1st Division, Department of Internal Medicine, Yokohama City University School of Medicine, Urafune-cho, Minami-ku, Yokohama 232, Japan;2. Tissue Culture Laboratory, Yokohama City University School of Medicine, Urafune-cho, Minami-ku, Yokohama 232, Japan;3. Department of Pharmacology, Nippon Dental College, Fujimi, Chiyoda-ku, Tokyo 102 Japan;1. School of Public Health, Georgia State University, Atlanta;2. Department of Kinesiology & Health, Georgia State University, Atlanta;3. Duke Cancer Institute, Duke University Medical Center, Durham, NC;4. Public Health Sciences, Wake Forest School of Medicine, Medical Center Boulevard, Winston–Salem, NC;1. Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden;2. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;1. College of Automation, Hangzhou Dianzi University, Hangzhou 310018, China;2. College of Accounting, Hangzhou Dianzi University, Hangzhou 310018, China;3. Center of Advanced Optoelectronic Materials and Devices, Key Laboratory of Novel Materials for Sensor of Zhejiang Province, College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China;4. College of Pharmacy, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China;5. Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China;6. Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, China |
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| Abstract: | Effect of sodium nitrite on cultured FM3A cells, a C3H mouse mammary carcinoma cell line, was examined. The chromosomal preparations demonstrated that severe aberrations were induced in more than 80% of the mitotic plates at 10−2 M and in nearly 40% at 10−2.5 M after 24 and 48 h treatment. According to the results of alkaline sucrose gradient analysis sedimentation profiles of cell DNA treated at as high as 10−1 M for 24 h scarcely changed from that of control cell DNA. Induction of 8-azaguanine-resistant mutation was demonstrated above 10−3 M sodium nitrite. |
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