Isolation and characterization of alpha-foetoprotein from foetal pigs |
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| Affiliation: | 1. Health Management Center, the Third Xiangya Hospital, Central South University, Changsha, China;2. Center for Experimental Medicine, the Third Xiangya Hospital, Central South University, Changsha, China;3. Disease Genome Research Center, Central South University, Changsha, China;4. Department of Neurology, the Third Xiangya Hospital, Central South University, Changsha, China;5. Department of Pathology, Changsha Maternal and Child Health Care Hospital, Changsha, China;6. Parkinson’s Disease Center and Movement Disorders Clinic, Department of Neurology, Baylor College of Medicine, Houston, TX, USA;1. M.M.Shemyakin–Y.A.Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation;2. A.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, 119991, Russian Federation;1. Department of Neuroscience and Mahoney Institute for Neurosciences, University of Pennsylvania, Philadelphia, PA 19104, USA;2. The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;3. Medical Scientist Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;4. Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA;5. Biochemistry and Molecular Biophysics Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA;6. Neuroscience Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA;7. Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;8. Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA;9. Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;10. Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;11. Bioengineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;12. Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;13. Corporal Michael J. Crescenz Veterans Affairs Medical Center, Philadelphia, PA 19104, USA;14. Glioblastoma Translational Center of Excellence, The Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA;15. Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;16. Department of Psychiatry, University of Pennsylvania, Philadelphia, PA 19104, USA;1. Department of Experimental Medicine, Faculty of Medicine, McGill University, Montreal, QC, Canada;2. Department of Developmental Neurobiology, St. Jude Children''s Research Hospital, Memphis, TN, 38105, USA |
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| Abstract: | - 1.1. Porcine α-foetoprotein, APP, was isolated from serum of pig foetuses using preparative electrophoresis and irnmunosorbent techniques.
- 2.2. The purity of the isolated AFP was controlled by polyacrylamide gel electrophoresis, electro-immunoassay and by injection of isolated AFP into rabbits.
- 3.3. During the preparation of AFP from foetal pig serum an unexpected α1-globulin appeared as a contaminating protein.
- 4.4. The molecular weight, isoelectric point and sedimentation coefficient were found to be 70,000, 4–61 and 4–56 respectively. No microheterogeneity as regards the physicochemical parameters investigated could be seen. The results were compared with those reported for some other mammals.
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