Isolation of sarcolemmal membranes from cardiac muscle |
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| Affiliation: | 1. Department of Medicine, Division of Cardiology, University of California San Diego, La Jolla, CA, USA;2. Department of Pharmacology, University of California San Diego, La Jolla, CA, USA;3. Department of Surgery, University of California San Diego, La Jolla, CA, USA;4. Cardiovascular Institute, Adult Medical Genetics Program, University of Colorado Denver, Aurora, CO, USA;5. Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplant, University of Colorado Denver, Aurora, CO, USA;6. Institute for Genomic Medicine, University of California San Diego, La Jolla, CA, USA;1. The School of Cardiovascular Medicine and Sciences, King''s College London British Heart Foundation Centre of Research Excellence, London SE5 9NU, UK;2. Developmental Biology, Laboratory Animal Science, The Institute of Experimental Animal Sciences, Osaka University Medical School, Suita 565-0871, Japan;3. Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan;4. Department of Genome Biology, Osaka University Graduate School of Medicine, Suita 565-0871, Japan |
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| Abstract: | - 1.1. Sarcolemmal membranes from cardiac tissue were isolated to a high degree of purity by brief extraction of homogenate with KC1 followed by 2 successive discontinuous sucrose density gradients of extracted particles.
- 2.2. Sarcolemmal membranes contained 40% adenylate cyclase, 22% guanylate cyclase and 20% ouabain-sensitive (Na+−K+) ATPase: contamination of sarcolemma by other intracellular membranes was negligible.
- 3.3. Electron microscopic examination of membranes showed presence of relatively empty vesicles of various shapes and sizes while electrophoretic analysis revealed about 20 protein bands of which 6 were prominent.
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