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Characterisation of abscisic acid inhibition of stomatal opening in isolated epidermal strips
Affiliation:1. State Key Laboratory of Earthquake Dynamics, Institute of Geology, China Earthquake Administration, Beijing 100029, China;2. Ocean University of China, Qingdao, Shandong Province 266100, China;1. Institute of Plant Bioenergetics, Faculty of Biology, University of Warsaw, I. Miecznikowa 01, 02-096 Warsaw, Poland;2. Departmentof Biology, Lund University, Sölvegatan 35B, SE-223 62 Lund, Sweden;1. Plant Gene Engineering Center, Chinese Academy of Sciences Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China;2. University of Chinese Academy of Sciences, Beijing, China;1. School of Computer and Information Science, Southwest University, Chongqing 400715, China;2. School of Hanhong, Southwest University, Chongqing 400715, China;3. Department of Industrial and Systems Engineering, The Hong Kong Polytechnic University, Hong Kong, China;4. School of Engineering, University of British Columbia, 1137 Alumni Ave, Kelowna, BC V1V 1V7, Canada;5. School of Engineering, Vanderbilt University, Nashville, TN 37235, USA;6. School of Automation, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China
Abstract:Abscisic acid (ABA) inhibited the light-induced opening of stomata in isolated epidermal strips of Commelina benghalensis. It did not alter stomatal closure in the dark. The ABA-induced inhibition in light was released under conditions conducive for cyclic photophosphorylation and remarkably reversed by ATP in the presence of pyruvate. Cyclic photophosphorylation rates of isolated guard cell chloroplasts were significantly reduced by ABA. It is proposed that the direct effect of ABA on stomatal opening was mediated in two ways: (1) by inhibition of cyclic photophosphorylation activities of guard cell chloroplasts and (2) by blocking organic acid formation in guard cells.
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