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Identification of JH III in haemolymph from adults and larvae of Schistocerca gregaria
Affiliation:1. Food Quality and Design Group, Wageningen University and Research, 6700AA, Wageningen, the Netherlands;2. Department of Food Science and Technology, Chinhoyi University of Technology, P Bag, 7724, Chinhoyi, Zimbabwe;1. Department of Biological Sciences, University of Cape Town, Rondebosch, 7701, South Africa;2. Centre for BioInnovation, University of the Sunshine Coast, Sippy Downs, Queensland, 4556, Australia;3. School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, 4556, Australia;1. KU Leuven, Department of Microbial and Molecular Systems (M2S), Lab4Food, Technology Campus Geel, Kleinhoefstraat 4, B-2440 Geel, Belgium;2. KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), Leuven, Belgium;3. Department of Food Technology and Nutrition, CAES, Makerere University, P.O. Box, 7062 Kampala, Uganda;1. Department of Entomology, Faculty of Science, Cairo University, P. O. Box 12613, Giza, Egypt;2. Key Lab for Biological Control of the Ministry of Agriculture, Department of Entomology, China Agricultural University, Beijing, 100193, PR China;3. Unité DGIMI UMR INRA-UM 1333, Université Montpellier, Place Eugène Bataillon, Montpellier, France;1. Norwegian University of Science and Technology (NTNU), Department of Electronic Systems, 7491 Trondheim, Norway;2. Norwegian University of Science and Technology (NTNU), Department of Energy and Process Engineering, 7491 Trondheim, Norway;3. SINTEF Energy Research, 7034 Trondheim, Norway
Abstract:An analytical method for the separation and identification of JH III and (E,E)-methyl farnesoate from locust haemolymph is described. Using this method, JH III was identified in haemolymph from adults, but no (E,E)-methyl farnesoate was found. JH III was also identified in fourth instar hopper haemolymph fractions, which had previously been shown to be active in the Galleria pupal wax assay.
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