| 蜂猴属（Nycticebus）D-loop控制区和Cyt b基因遗传学研究 |
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| 引用本文： | 朱万龙,章迪,高文荣,王政昆. 蜂猴属（Nycticebus）D-loop控制区和Cyt b基因遗传学研究[J]. 生物学杂志, 2014, 0(5): 1-5 |
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| 作者姓名： | 朱万龙 章迪 高文荣 王政昆 |
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| 作者单位： | 云南师范大学生命科学学院,昆明650500 |
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| 基金项目： | 国家国际科技合作项目（No.2014DFR31040）;十二五国家支撑计划（No.2014BA101B00）;国家自然科学基金资助项目（No.31360096）;云南省应用基础研究项目（No.2013FA014） |
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| 摘 要： | 测定了蜂猴属线粒体DNA中的D-loop控制区部分序列和细胞色素b基因全序列（1140 bp），对其变异情况进行了分析，并采用Mega 4.0软件中构建了分子系统树。无论是基于D-loop控制区部分序列构建的系统树，还是基于细胞色素b基因序列构建的分子系统树的拓扑结构图，都清晰地表明蜂猴属由两个分支组成，分支置信度较高，一支由N．pygmaeus聚成，另一支由N．coucang聚成，即支持蜂猴属由N．coucang和N．pygmaeus两物种组成。
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| 关 键 词： | 倭蜂猴 蜂猴 线粒体DNA 分子遗传学 |
Molecular genetics of Nycticebus inferred from D-loop control region and cytochrome b gene |
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| ZHU Wan-long,ZHANG Di,GAO Wen-rong,WANG Zheng-kun. Molecular genetics of Nycticebus inferred from D-loop control region and cytochrome b gene[J]. Journal of Biology, 2014, 0(5): 1-5 |
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| Authors: | ZHU Wan-long ZHANG Di GAO Wen-rong WANG Zheng-kun |
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| Affiliation: | (School of Life Sciences, Yunnan Normal University, Kunming 650500, China) |
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| Abstract: | To understand the phylogeny and classification of Nycticebus, the partial D-loop （400 bp） and cytochrome b （ Cyt b, 1140 bp） genes from all recognized taxa in Nycticebus were sequenced.A molecular phylogeny was established by using Mega 4.0.Whether analysis combining D-loop sequences data with complete sequences of mitochondrial cytochrome b gene data performed or analysis em-ployed each of them separately, all of derived results revealed that genus Nycticebus is comprised of two monophyletic Glades which well supported by high bootstrap value.The first cluster comprised all individuals of N.pygmaeus;the second cluster was grouped with all individuals of N.coucang, which support the genus Nycticebus was composited with N.coucna g and N.pygmaeus. |
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| Keywords: | Nycticebu pygmaeus Nycticebvz coucang mitochondrial DNA molecular genetics |
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