| 山西霍山五角枫不同海拔种群遗传多样性研究 |
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| 引用本文： | 张翠琴,林丽丽,王祎玲. 山西霍山五角枫不同海拔种群遗传多样性研究[J]. 生物学杂志, 2014, 0(5): 66-70 |
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| 作者姓名： | 张翠琴 林丽丽 王祎玲 |
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| 作者单位： | 山西师范大学生命科学学院,临汾041004 |
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| 基金项目： | 山西师范大学大学生创新性实验项目（SD2011CXSY-21） |
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| 摘 要： | 利用SRAP分子标记分析山西霍山不同海拔五角枫种群的遗传多样性和遗传结构。11对引物组合共得到88个重复性好的位点；Nei基因多样性指数（ h）在0.1996～0.3163之间，平均为0.2373；Shannon多样性指数（ I）范围为0.2995～0.4936，平均为0.3535；在物种水平上，多态位点百分率（ PPB）为98.86％，表现出较高地遗传多样性。 ANOVA分析表明，遗传变异主要存在于五角枫种群内（79％），21％的分子变异分布在种群间。基于遗传距离进行UPGMA聚类分析，5个五角枫种群聚为明显的2支。相关分析显示：五角枫的多样性指数与地理梯度均无显著或极显著相关。
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| 关 键 词： | 霍山 五角枫 遗传多样性 SRAP |
Genetic diversity of Acer mono maxim populations at different altitude in Huoshan,Shanxi |
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| ZHANG Cui-qin,LIN Li-li,WANG Yi-ling. Genetic diversity of Acer mono maxim populations at different altitude in Huoshan,Shanxi[J]. Journal of Biology, 2014, 0(5): 66-70 |
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| Authors: | ZHANG Cui-qin LIN Li-li WANG Yi-ling |
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| Affiliation: | (College of Life Science, Shanxi Normal University, Linfen 041004, China) |
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| Abstract: | To estimate genetic diversity and genetic structure of Acer mono maxim at different elevations, five natural populations were surveyed by using SRAP markers.A total of 11 pairs of SRAP primer combinations amplified 88 bands.Nei′s gene diversity （ h） and Shannon’ s information index （ I） were ranged from 0.1996 to 0.3163 and from 0.2995 to 0.4936, respectively.At species level, the percentage of polymorphic loci of Acer mono maxim （ PPB） was 98.86%, indicating a high genetic diversity occurred within Acer mono maxim.ANOVA showed that the genetic variation mainly existed within populations （79%） and only 21%among populations.Based on the genetic distance between populations, five populations mainly clustered into two groups.Correlation analysis revealed the rela-tionship between different diversity indices and soil chemical properties without significant or very significant correlation. |
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| Keywords: | Huoshan Acer motto maxim genetic diversity SRAP |
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