| 基于 AOPAN 纳米纤维膜的粘杆菌素发酵液后处理研究 |
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| 引用本文： | 凤权,;魏安静,;侯大寅,;武丁胜.基于 AOPAN 纳米纤维膜的粘杆菌素发酵液后处理研究[J].生物学杂志,2014(5):89-92. |
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| 作者姓名： | 凤权 ;魏安静 ;侯大寅 ;武丁胜 |
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| 作者单位： | [1]安徽工程大学纺织面料安徽省高校重点实验室,安徽芜湖241000; [2]安徽工程大学安徽省电气传动与控制重点实验室,安徽芜湖241000 |
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| 基金项目： | 国家自然科学基金（21377004）;安徽省自然科学基金（编号：1408085ME87）;安徽省高校自然科学研究项目（编号：KJ2012A039、KJ20128014） |
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| 摘 要： | 使用静电纺丝再经胺肟化改性制备AOPAN纳米纤维膜，并基于AOPAN纳米纤维膜构建膜分离装置，用于粘杆菌素发酵液的后处理。研究中，对不同厚度的纳米纤维膜的渗透通量及分离性能进行比较，最终确定双层叠合的纳米纤维膜为分离膜，最适操作压力为0.14 MPa。在此条件下，分离膜的渗透通量为2.61 L/m2．min，蛋白质的截留率达到90％，色素等其他杂质也得到有效去除。
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| 关 键 词： | 发酵液 AOPAN 纳米纤维膜 过滤 |
Study on preparation of AOPAN nanofibrous membrane and their applications for filtration treatment of colistin fermentation broth |
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| Institution: | FENG Quan, WEI An-jing, HOU Da-yin, WU Ding-sheng ( 1. Anhui Provincial Key Laboratory of Textile Fabric ; 2. Anhui Provincial Key laboratory of Electric and Control, Anhui Polytechnic University, Wuhu 241000, China ) |
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| Abstract: | Polyacryonitrile （ PAN） nanofibers were prepared by electrospinning, and amidoximepolyacryonitrile （ AOPAN） nanofibrous membranes were generated by chemical modification of PAN nanofibers, which were used as the filtration membrane for separation of colistin fermentation broth.According to the experimental data, characters materials and comparing nanofibrous membranes filtration separation performance, double-layer nanofibrousmembrane was selected.The optimum operating parameters had been determined：pressure was 0.14 Mpa.Under these optimum parameters, the flux of the nanofibrous membrane was 2.61 L/m2 .min, the protein reta-tion ratio was is nearly 90%, and the part of pigment and other impurities had been removed. |
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| Keywords: | fermentation broth AOPAN nanofibrous membrane filtration |
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