Three members of the S multigene family are linked to the S locus of Brassica |
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Authors: | G Suzuki M Watanabe N Kai N Matsuda K Toriyama S Takayama A Isogai and K Hinata |
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Institution: | (1) Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 1–1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981, Japan. Fax: +81-22-717-8654. e-mail: nabe@bios.tohoku.ac.jp, JP;(2) Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630–01, Japan, JP;(3) Research Institute of Seed Production Co., Ltd., Sendai 989–32, Japan, JP |
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Abstract: | Two self-incompatibility genes in Brassica, SLG and SRK (SLG encodes a glycoprotein; SRK encodes a receptor-like kinase), are included in the S multigene family. Products of members of the S multigene family have an SLG-like domain (S domain) in common, which may function as a receptor. In this study, three clustered
members of the S multigene family, BcRK1, BcRL1 and BcSL1, were characterized. BcRK1 is a putative functional receptor kinase gene expressed in leaves, flower buds and stigmas, while BcRL1 and BcSL1 are considered to be pseudogenes because deletions causing frameshifts were identified in these sequences. Sequence and expression
pattern of BcRK1 were most similar to those of the Arabidopsis receptor-like kinase gene ARK1, indicating that BcRK1 might have a function similar to that of ARK1, in processes such as cell expansion or plant growth.
Interestingly, the region containing BcRK1, BcRL1 and BcSL1 is genetically linked to the S locus and the physical distance between SLG, SRK and the three S-related genes was estimated to be less than 610 kb. Thus the genes associated with self-incompatibility exist within a cluster
of S-like genes in the genome of Brassica.
Received: 15 April 1997 / Accepted: 13 June 1997 |
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Keywords: | Self-incompatibility Brassica campestris Receptor-like kinase S multigene family Gene cluster |
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