A RAPD-PCR method for the rapid detection of Bacillus cereus |
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| Authors: | Lee Jiyeon Kwon Gun-Hee Park Jae-Yong Park Cheon-Seok Kwon Dae Young Lim Jinkyu Kim Jong Sang Kim Jeong Hwan |
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| Institution: | Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju, Korea. |
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| Abstract: | Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample. |
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