Analysis of heterogeneity of human keratinocytes interacting with immobilized fibronectin and collagenes of types I and IV |
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Authors: | O G Spichkina G P Pinaev Y P Petrov |
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Institution: | (1) Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia |
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Abstract: | Basing on the natural affinity of skin keratinocytes toward extracellular matrix proteins, we have attempted to dissect the
population of these cells by varying the time of their adhesion to substrates from fibronectin and collagen of types I and
IV. After selection for 10, 20, and 30 min, the keratinocytes were cultivated for 24 h under standard conditions. The area
of cell projection on the substrate and the spreading coefficient were measured. Statistically significant morphological differences
between cells selected on different substrates were found. The size of cells growing on type-I collagen was twice as large
as that of the cells cultivated on collagen type-IV or on fibronectin. Independent of the substratum, up to 60–65% of the
cells had a round shape. Keratinocytes cultivated on collagens revealed heterogeneity both in the control and after selection
in their adhesion times, while the cells grown on fibronectin behaved as a homogeneous population. These results suggest that,
contrary to fibronectin, collagens stabilize some physiological states of keratinocytes corresponding to their interactions
with extracellular matrix proteins in the organism.
Original Russian Text O.G. Spichkina, G.P. Pinaev, Y.P. Petrov, 2008, published in Tsitologiya, Vol. 50, No. 2, 2008. |
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Keywords: | adhesion heterogeneity of cell populations human keratinocytes collagen fibronectin |
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