Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target |
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Authors: | Ekström Simon Wallman Lars Hök Daniel Marko-Varga György Laurell Thomas |
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Institution: | Department of Electrical Measurements, Lund Institute of Technology, Lund University, Lund, Sweden. |
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Abstract: | A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides. |
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