The metabolic effects and uptake of ethidium bromide by rat liver mitochondria. |
| |
Authors: | A. Peña E. Chávez A. Cárabez M.Tuena De Gómez-Puyou |
| |
Affiliation: | Departamento de Biología Experimental, Instituto de Biología, Universidad Nacional Autónoma de México, Apartado Postal 70-600, México 20, D. F. Mexico |
| |
Abstract: | The uptake of ethidium bromide by rat liver mitochondria and its effect on mitochondria, submitochondrial particles, and F1 were studied. Ethidium bromide inhibited the State 4-State 3 transition with glutamate or succinate as substrates. With glutamate, ethidium bromide did not affect State 4 respiration, but with succinate it induced maximal release of respiration. These effects appear to depend on the uptake and concentration of the dye within the mitochondrion. In submitochondrial particles, the aerobic oxidation of NADH is much more sensitive to ethidium bromide than that of succinate. Ethidium bromide partially inhibited the ATPase activity of submitochondrial particles and of a soluble F1 preparation. Ethidium bromide behaves as a lipophilic cation which is concentrated through an energy-dependent process within the mitochondria, producing its effects at different levels of mitochondrial function. The ability of mitochondria to concentrate ethidium bromide may be involved in the selectivity of the dye as a mitochondrial mutagen. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|