A new strain of <Emphasis Type="Italic">Streptomyces avermitilis</Emphasis> produces high yield of oligomycin A with potent anti-tumor activity on human cancer cell lines in vitro |
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Authors: | Xiuping Lin Ying Wen Meng Li Zhi Chen Jia Guo Yuan Song Jilun Li |
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Institution: | (1) State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China, 100193 |
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Abstract: | A new actinomycete strain, isolated from soil in China, strongly inhibited in vitro proliferation of human hepatoma, chronic
myelogenous leukemia, and colonic carcinoma cell lines. The strain, designated L033, was identified as a strain of Streptomyces avermitilis based on cultural property, morphology, carbon source utilization, 16s rRNA gene analysis, and DNA–DNA relatedness studies.
The anticancer component from L033 was purified to homogeneity by preparative positive-phase high-performance liquid chromatography
and crystallization. Nuclear magnetic resonance and mass spectrometric analysis showed that this compound had the same structure
as oligomycin A. Different with other reported naturally occurring strains of S. avermitilis, L033 produced high quantity of oligomycin A (maximal 1,461 μg/ml). Therefore, L033 was considered of great potential as
an industrial oligomycin-A-producing strain.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Streptomyces avermitilis Oligomycin A Anti-tumor activity |
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