Signal transduction pathways in the induction of HLA class I antigen expression on Huh 6 cells by interferon-gamma. |
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| Authors: | T Towata N Hayashi K Katayama T Takehara Y Sasaki A Kasahara H Fusamoto T Kamada |
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| Institution: | First Department of Medicine, Osaka University Medical School, Japan. |
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| Abstract: | This study investigated the intracellular signal transduction regulating the appearance of HLA class I antigens on Huh 6 cells induced by interferon-gamma. The expression was blocked by a protein kinase C inhibitor, H-7, but not by a calmodulin antagonist, W-7, nor by a protein kinase A inhibitor, H-8, at low dose. The antigen expression was induced by a direct activator of protein kinase C, phorbol myristate acetate, but not by calcium ionophore A23187 nor an analog of cAMP, dbcAMP. Therefore, we concluded that protein kinase C is involved in the expression of HLA class I antigens on Huh 6 cells induced by interferon-gamma but Ca(2+)-calmodulin and cAMP are not. |
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