Regional distribution of in vitro release of thyrotropin releasing hormone in rat brain |
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| Authors: | M Mendez P Joseph-Bravo M Cisneros M A Vargas J L Charli |
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| Institution: | 1. Grupo de Biotecnología y Materiales Biobasados, Instituto de Tecnología en Polímeros y Nanotecnología (ITPN-UBA-CONICET), Facultad de Ingeniería, Universidad de Buenos Aires, Las Heras 2214 (CP 1127AAR), Buenos Aires, Argentina;2. Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Argentina (UBA), Av. Intendente Güiraldes 2620 (CP 1428BGA), Pabellón de Industrias, Ciudad Universitaria, Buenos Aires, Argentina;3. Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB), Hospital Italiano de Buenos Aires (HIBA), CONICET, Instituto Universitario HIBA, Potosí 4240 (CP 1199), Buenos Aires, Argentina;4. Centro IREN, Universidad Tecnológica Nacional, Buenos Aires, Argentina;5. Grupo de Ingeniería en Polímeros y Materiales Compuestos, Instituto de Tecnología en Polímeros y Nanotecnología (ITPN-UBA-CONICET), Facultad de Ingeniería, Universidad de Buenos Aires, Las Heras 2214 (CP 1127AAR), Buenos Aires, Argentina;6. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;7. Instituto de Ingeniería Biomédica, Universidad de Buenos Aires, Buenos Aires, Argentina;8. Department of Electrical and Computer Engineering, Florida International University, Miami, FL 33174, USA;1. Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras 37200-000, Minas Gerais State, Brazil;2. Instituto Tecnológico Vale, Belém 66055-090, Pará State, Brazil;3. Departamento de Química, ICEX, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais State, Brazil;1. VA Puget Sound Health Care System, Office of Research and Development, Medical Research Service, Department of Veterans Affairs Puget Sound Health Care System, Seattle, WA 98108, USA;2. Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA |
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| Abstract: | To increase our knowledge of the TRH functions in brain and the processes of TRH compartmentalization and release, we studied the in vitro release of endogenous TRH in different brain areas. We also determined the correlation between TRH levels and release under both basal and stimulated conditions. TRH concentration was measured in tissues and media by specific radioimmunoassay. TRH-like material detected in olfactory bulb and hypothalamic incubates (basal or K+ stimulated) were shown to be chromatographically identical to synthetic TRH. Different brain regions showed high variability in the basal release of TRH (1-20% of tissue content). This suggests the existence of different pools. The response to depolarizing stimulus (56 mM K+) was significant only in the following regions: median eminence, total hypothalamus, preoptic area, nucleus accumbens-lateral septum, amygdala, mesencephalon, medulla oblongata and the cervical region of the spinal cord. These regions have been shown to contain a high number of receptors, a high concentration of TRH nerve endings and are susceptible to TRH effects. These results support the hypothesis that TRH functions as neuromodulator in these areas. |
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