Investigation of various structures of DNA molecules (III) |
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Authors: | Xizeng Feng Zhang Lin Chen Wang Chunli Bai |
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Institution: | (1) National Center for Surfactant Research and Chemical Engineering, Research Institute of Daily Chemical Industry, 030001 Taiyuan, China;(2) School of Chemistry and Chemical Engineering, Taiyuan University of Technology, 030002 Taiyuan, China; |
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Abstract: | The structure transition of λ-DNA induced by cationic surfactant cellar media was investigated by using CD, SEM and AFM. The experimental data of CD revealed that λ-DNA can be induced from B-form to a collapsed structure with the addition of the cationic surfactant CTAB to the system. The condensed process of λ-DNA from coil state to small globular state (diameter about 1.25 μm) and finally big globular state (diameter about 5.4 μm) was observed by using SEM and AFM. |
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