Design and synthesis of a novel DNA-encoded chemical library using Diels-Alder cycloadditions |
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Authors: | Buller Fabian Mannocci Luca Zhang Yixin Dumelin Christoph E Scheuermann Jörg Neri Dario |
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Affiliation: | Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland. |
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Abstract: | DNA-encoded chemical libraries are increasingly being employed for the identification of binding molecules to protein targets of pharmaceutical relevance. Here, we describe the synthesis and characterization of a DNA-encoded chemical library, consisting of 4000 compounds generated by Diels-Alder cycloaddition reactions. The compounds were encoded with unique DNA fragments which were generated through a stepwise assembly process and serve as amplifiable bar codes for the identification and relative quantification of library members. |
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