| 白刺金琥组织培养研究 |
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| 引用本文： | 林荔琼,刘景春.白刺金琥组织培养研究[J].亚热带植物科学,2002,31(3):71-73. |
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| 作者姓名： | 林荔琼 刘景春 |
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| 作者单位： | 厦门市农业科学研究所, 福建 厦门 361009 |
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| 摘 要： | 以白刺金琥种子无菌播种产生的小球体为外植体,进行愈伤组织诱导和小球分化。结果表明,去除顶端生长点的小球在MS+6-BA 1.0mg/L+NAA 0.1mg/L的培养基中,可由刺座直接产生大量小球体,而切口形成的愈伤组织在6-BA浓度低于4.0mg/L时无法分化小球,当6-BA浓度提高到4.0~6.0mg/L时,亦可分化小球体,但诱导率低且周期长。继代培养在MS+6-BA 0.5 mg/L+NAA 0.1mg/L培养基中进行,单球在1/2MS加或不加NAA的培养基上均可生根。
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| 关 键 词： | 白刺金琥 组织培养 |
| 文章编号： | 1009-7791(2002)03-0071-03 |
| 收稿时间： | 2002-05-13 |
| 修稿时间： | 2002年5月13日 |
Study on tissue culture of Echinocactus grusonii var. albispinus |
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| LIN Li-qiong,LIU Jing-chun.Study on tissue culture of Echinocactus grusonii var. albispinus[J].Subtropical Plant Science,2002,31(3):71-73. |
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| Authors: | LIN Li-qiong LIU Jing-chun |
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| Institution: | Xiamen Institute of Agricultural Science, Xiamen 361009, Fujian China |
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| Abstract: | Taking the spheroids that produced from seeds in bacteria free environment as explants, tissue culture of Echinocactus grusonii var. albispinus was conducted. The results showed that the spheroids could differentiate into substantial new spheroids on MS+6-BA 1.0mg/L+NAA 0.1mg/L when the apical point of explants were removed. When the concentration of 6-BA was 4.0~6.0mg/L, the callus differentiation could be induced, but the rate of induction was low with longer period. The optimal medium for subculture was MS+6-BA 0.5mg/L+NAA 0.1mg/L and 1/2MS with or without NAA was best for rooting. |
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| Keywords: | Echinocactus grusonii var albispinus tissue culture |
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