Amino acid sequence of porcine heart fumarase |
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| Authors: | J C Sacchettini M W Frazier D C Chiara L J Banaszak G A Grant |
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| Institution: | Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110. |
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| Abstract: | The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure. |
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