Survival of bifidobacteria administered to calves |
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Authors: | E Vlková M Grmanová J Killer J Mrázek J Kope?ny V Bune?ová and V Rada |
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Institution: | (1) Food Research and Development Centre, Agriculture Canada, Saint Hyacinthe, Quebec, Canada;(2) Departments of Animal Science and Food Science and Nutrition, Laval University, Quebec City, Quebec, Canada;(3) School of Dietetics and Human Nutrition, McGill University, Montreal, Quebec, Canada |
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Abstract: | Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and
antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were
prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored
in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered
bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation
using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora. Significantly lower amounts of E. coli and higher amounts of bifidobacteria and total anaerobes were found in the treated group relative to the control group. |
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