Highly hydroxylated or γ-cyclodextrin-bicapped water-soluble derivative of fullerene: The antioxidant ability assessed by electron spin resonance method and β-carotene bleaching assay |
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Authors: | Shinya Kato Hisae Aoshima Yasukazu Saitoh Nobuhiko Miwa |
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Institution: | aLaboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima 727-0023, Japan;bVitamin C60 BioResearch Corporation, Tatsunuma Tatemono Bldg. 9F, 1-3-19 Yaesu Chuo-ku, Tokyo 104-0031, Japan |
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Abstract: | Antioxidant ability of the water-soluble derivative of fullerene (C60), prepared by high-degree hydroxylation C60-(OH)32·8H2O] or C60/γ-cyclodextrin (1:2 mol/mol) clathrate formation C60/(γ-CD)2], was assessed by electron spin resonance method and β-carotene bleaching assay. These C60 derivatives have an ability to diminish a 1:2:2:1 quartet ESR spectrum attributed to hydroxyl radicals ( OH) as shown by DMPO-spin trap/ESR method. Meanwhile, a singlet radical-signal different from OH-attributed signals increased in a manner dependent on concentrations of C60-(OH)32·8H2O. This might suggest that C60-(OH)32·8H2O scavenges OH owing to dehydrogenation of C60-(OH)32·8H2O, and is simultaneously oxidized to a stable radical species, which may be a dehydrogenated fullerenol radical (C60-O ). Furthermore, these water-soluble derivatives of C60 suppressed fading of yellowish color characteristic of intact β-carotene in β-carotene bleaching assay. Antioxidant abilities of these derivatives were assessed as retention of yellowish color (viz absorbance at 470 nm) for 180 min. Namely, β-carotene-attributed chromaticity (% relative absorbance at 470 nm compared with the control) after 180 min was 69% for C60-(OH)32·8H2O (400 μM: C60-eq.), and 32% for C60/(γ-CD)2 (400 μM: C60-eq.), whereas it was 6% for l(+)-ascorbic acid (400 μM) which is hydrophilic, and 85% for (±)-α-tocopherol (400 μM) which is lipophilic, respectively. Thus C60-(OH)32·8H2O and C60/(γ-CD)2 can scavenge OH, and have a distinct antioxidative activity in the aqueous system containing linoleic acid which is abundantly contained in the cell membrane together with other unsaturated lipids. These C60 derivatives have a potential to protect the cell membrane from oxidative stress due to OH. |
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Keywords: | Water-soluble fullerene Antioxidant ability Electron spin resonance (ESR) method β -Carotene bleaching assay |
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