An autoradiographic study of macromolecular syntheses in the epithelium of the ductus epididymidis in the mouse |
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Authors: | V Kope?ný V Pech |
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Institution: | (1) Institute of Physiology and Genetics of Animals, Czechoslovak Academy of Sciences, CS-277 21 Libchov, Czechoslovakia |
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Abstract: | Summary Glycoprotein dynamism in the mouse epididymis was studied by means of histoautoradiography after injection of l-fucose-1-3H. The label was detected, at thirty minutes p.i., in the area occupied by Golgi apparatus in the epithelial cells. At 4 h p.i. the label was already present in the lumen of ductus epididymidis. At this time interval, the luminal labelling was highest in the initial segment of the epididymis and decreased against the more distal segments considerably.At ten days p.i. very high labelling was detected in the luminal contents in the terminal segment of the ductus epididymidis and in ductus deferens, the labelling in the proximal segments of the epididymis being much lower. These observations suggested a wave of labelled glycoprotein in epididymal plasma passing through the epididymis after a fucose pulse.Higher labelling was detected in so-called clear cells than in the neighboring principal cells.Association of the labelled material was seen in epididymal and uterine spermatozoa, mostly in sperm tail region. |
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