Microcalorimetric investigation on the growth model and the protein yield of Bacillus thuringiensis |
| |
Authors: | Xiaoyan Lin Yi Liu Peng Liu Songsheng Qu Ziniu Yu |
| |
Institution: | Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Life Science and Technology College, Huazhong Agriculture University, Wuhan 430070, Hubei, PR China. linxiaoyan@hotmail.com |
| |
Abstract: | A novel microcalorimetric technique based on the bacterial heat output was applied to evaluate the special growth model, the protein expression and the generation time of Bacillus thuringiensis for the first time. The thermogenic curves of the aerobic metabolism of B. thuringiensis strains YBT-833, YBT-1520 and YBT-833-2-1 were determined by using an LKB-2277 BioActivity Monitor. The analysis of the thermogenic curves indicated both the mutant strain and the wild-type strains followed the same linear growth model during sporulation. The metabolism heat output revealed heat output was correlated to the yield of the insecticidal crystal proteins (ICPs) very well, the more protein product, and the less heat output. Based on the data acquired, we proposed that this method could be a useful tool in monitoring the fermentation of B. thuringiensis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|