Kinetics of growth and fatty acid production of Rhodotorula glutinis |
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Authors: | L -M Granger P Perlot G Goma A Pareilleux |
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Institution: | (1) Centre de Transfert en Biotechnologie Microbiologie, Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31007 Toulouse Cedex, France;(2) D.R.D. Béghin Say (Gruppo Ferruzzi), 54 Avenue Hoche, F-75008 Paris Cedex 08, France |
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Abstract: | Summary The effect of culture conditions, especially nitrogen/carbon (N/C) ratio and temperature, on acyl lipid profiles in the oleaginous yeast Rhodotorula glutinis NRRL Y 1091 have been investigated. Cultivation of the microorganism under N-limited conditions (N/C < 0.14 g/g) resulted in enhanced fatty acid (FA) cell content but a reduced relative amount of polyunsaturated fatty acids (PUFA). The maximal FA productivity was obtained for a 0.025 N/C ratio resulting from the arrangement between the specific rate of FA synthesis and the concentration of lipid-free biomass. Under nitrogen-non-limiting conditions, cells grown at lower temperatures had a higher PUFA content and the maximal productivity of -linolenic acid was obtained by shifting the temperature of the culture from 30° C to 25° C.
Offprint requests to: A. Pareilleux |
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