The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells |
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Authors: | Matthew?D?Hall Carolyn?T?Dillon Mei?Zhang Philip?Beale Zhonghou?Cai Barry?Lai Anton?P?J?Stampfl Email author" target="_blank">Trevor?W?HambleyEmail author |
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Institution: | (1) Centre for Heavy Metals Research, School of Chemistry F11, The University of Sydney, 2006, N.S.W. , Australia;(2) Department of Radiation Oncology, Royal Prince Alfred Hospital, 2050 Camperdown, N.S.W. , Australia;(3) Department of Medical Oncology, Sydney Cancer Centre, Royal Prince Alfred Hospital, 2050 Camperdown, N.S.W. , Australia;(4) Experimental Facilities Division, Argonne National Laboratory, Argonne, Il 60439, USA;(5) Physics Division, Australian Nuclear Science and Technology Organisation, 2234 Lucas Heights, N.S.W. , Australia |
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Abstract: | The cellular distribution of platinum in A2780 ovarian cancer cells treated with cisplatin and platinum(IV) complexes with a range of reduction potentials has been examined using elemental analysis (synchrotron radiation-induced X-ray emission). The cellular distribution of platinum(IV) drugs after 24 h is similar to that of cisplatin, consistent with the majority of administered platinum(IV) drugs being reduced. Micro-X-ray absorption near-edge spectra of cells treated with cisplatin and platinum(IV) complexes confirmed the reduction of platinum(IV) to platinum(II). In cells treated, the most difficult to reduce complex, cis,trans,cis-PtCl2(OH)2(NH3)2], platinum(IV) was detected in the cells along with platinum(II). The observations are in accordance with the relative ease of reduction of the platinum(IV) complexes used and support the requirement of reduction for activation of platinum(IV) complexes.Abbreviations en ethane-1,2-diamine - GM growth medium - PBS phosphate buffered saline - RPMI Roswell Park Memorial Institute - SRIXE synchrotron radiation-induced X-ray emission - XAFS X-ray absorption fine structure - XANES X-ray absorption near-edge spectroscopy |
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Keywords: | Cellular distribution Cisplatin Ovarian cancer cells Platinum complexes Synchrotron radiation-induced X-ray emission |
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