Properties of inwardly rectifying K+ channels in ventricular myocytes |
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Authors: | Ira R Josephson |
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Institution: | (1) Department of Physiology and Biophysics, Wright State University, Dayton, Ohio, USA;(2) Present address: Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, 45267-0576 Cincinnati, OH, USA |
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Abstract: | Summary The voltage- and time-dependent properties of whole-cell, multi-channel (outside-out), and single channel inwardly-rectifying K+ currents were studied using adult and neonatal rat, and embryonic chick ventricular myocytes. Inward rectification of the current-voltage relationship was found in the whole-cell and single channel measurements. The steady-state single channel probability of opening decreased with hyperpolarization from EK, as did the mean open time, thereby explaining the time-dependent inactivation of the macroscopic current. Myocytes dialysed with a Mg++-free K+ solution (to remove the property of inward rectification) displayed a quasi-linear current-voltage relationship. The outward K+ currents flowing through the modified inward rectifier channels were able to be blocked by the local anesthetic and anti-arrhythmic agent, lidocaine. |
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Keywords: | ventricular myocytes patch clamp K+ channels inward rectification |
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