Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection |
| |
Authors: | Alison L Dann Calum R Wilson |
| |
Institution: | (1) Tasmanian Institute of Agricultural Research (TIAR), University of Tasmania, New Town Research Laboratories, 13 St Johns Ave, New Town, TAS, 7008, Australia; |
| |
Abstract: | Three long-term nodal tissued cultured Russet Burbank potato clones and nine thaxtomin A-treated regenerant lines, derived
from the nodal lines, were assessed for genetic and epigenetic (in the form of DNA methylation) differences by AFLP and MSAP.
The treated regenerant lines were originally selected for superior resistance to common scab disease and acceptable tuber
yield in pot and field trials. The long-term, tissue culture clone lines exhibited genetic (8.75–15.63% polymorphisms) and
epigenetic (12.56–26.13% polymorphisms) differences between them and may represent a stress response induced by normal plant
growth disruption. The thaxtomin A-treated regenerant lines exhibited much higher significant (p < 0.05) genetic (2–29.38%) and epigenetic (45.22–51.76%) polymorphisms than the nodal cultured parent clones. Methylation-sensitive
mutations accumulated within the regenerant lines are significantly correlated (p < 0.05) to disease resistance. However, linking phenotypic differences that could be of benefit to potato growers, to single
gene sequence polymorphisms in a tetraploid plant such as the potato would be extremely difficult since it is assumed many
desirable traits are under polygenic control. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|