Growth of human skin fibroblasts in dialyzed fetal bovine serum |
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Authors: | Eugene Elmore Michael Swift |
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Institution: | (1) Biological Sciences Research Center 220H, Genetics Curriculum, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina |
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Abstract: | Summary Human diploid fibroblast cultures plated at or below a density of 2×103 cells per cm2 grew very slowly or not at all in MEM supplemented with 10% fetal bovine serum that had been dialyzed for 24 hr. Adding serine
(0.2 mM) or pyruvate (1.0 mM) to MEM and 10% dialyzed serum restored growth to the level observed with 10% nondialyzed serum.
Serine and pyruvate also were able to overcome partially the growth arrest induced by a reduced serum concentration (1 or
2%). Human fibroblast cultures grew very well in 100% fetal bovine serum that had been dialyzed against MEM. For cells grown
in dialyzed serum, the final number increased with increasing serum concentration, in contrast to the well established toxic
effects of high concentrations of nondialyzed serum.
This research was supported by NIH Grants CA15207 and HD03110. |
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Keywords: | human fibroblasts serum toxicity dialysis |
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