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DNA amplification occurs between the core region of replicationori + of the ts plasmid integrated in the chromosome and its analog in the bacterial genome
Authors:F K Khasanov  V I Bashkirov
Institution:(1) Institute of Gene Biology, Russian Academy of Sciences, 117334 Moscow, Russia
Abstract:Integrated in theBacillus subtilis chromosome, hybrid plasmid pGG10 is capable of thermosensitive amplification. One amplification site corresponds to the core region of replicationori + of pE194, a component of pGG10; the other is a homologous region of theB. subtilis chromosome. A model of illegitimate amplification mediated by pE194 RepF is proposed.
Keywords:Bacillus subtilis  DNA amplification  plasmid pE194
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