Genomic organization of multiple genes coding for rhodotorucine A,a lipopeptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides |
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| Authors: | R. Akada J. Kai I. Yamashita T. Miyakawa S. Fukui |
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| Affiliation: | (1) Center for Gene Science, Faculty of Engineering, Hiroshima University, Saijo, 724 Higashi-Hiroshima, Japan;(2) Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo, 724 Higashi-Hiroshima, Japan;(3) Present address: Department of Biotechnology, Faculty of Technology, Fukuyama University, Higashimura-machi, 729-02 Fukuyama, Japan |
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| Abstract: | Rhodotorucine A, a lipopeptide mating pheromone, is secreted from mating type A cells of Rhodosporidium toruloides and induces sexual differentiation of the opposite mating type a cells. Genome of A-type cells contains three homologous genes (RHA1, RHA2, and RHA3) encoding rhodotorucine A. Genomic Southern blot analysis using RHA1 DNA as a probe showed that RHA1 strongly hybridize with A-type genomic DNA but weakly with a-type, suggesting that the sequences of RHA genes were dissimilar in the opposite a-type genome. The range of dissimilar regions in a-type genome was searched using RHA-flanking DNA segments as probes. The result suggests that a-type genome lacks sequences coding for rhodotorucine A and its 5 upstream but contains its 3 non-coding sequences. The absence of mating pheromone genes in the opposite mating type genome suggests that the expression of mating-type-specific genes in R. toruloides is not controlled trans-criptionally, as shown in the yeast Saccharomyces cerevisiae. |
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| Keywords: | Basidiomycetous yeast Rhodosporidium toruloides Rhodotorucine A Mating pheromone gene Genomic organization Mating types |
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