Heterogeneity of Methicillin-Resistant <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> Strains (MRSA) Characterized by Flow Cytometry |
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| Authors: | Tomasz Jarzembowski Katarzyna Wiśniewska Agnieszka Jóźwik Jacek Witkowski |
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| Institution: | (1) Department of Medical Microbiology, Medical University of Gdańsk, Str.Do Studzienki 38, 80-227 Gdańsk, Poland;(2) Department of Pathophysiology, Medical University of Gdańsk, Str.Do Studzienki 38, 80-227 Gdańsk, Poland |
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| Abstract: | We used fluorescent penicillin Bocillin FL for characterization of control methicillin-resistant Staphylococcus aureus (MRSA) strains belonging to one of four heterogenic classes and comparing them with clinical MRSA isolates. Significant differences
in percentage of fluorescent cells and reduction of Bocillin FL binding after incubation with methicillin between control
strains from classes I and IV were observed, whereas the strains from classes II and III were differed after incubation with
methicillin. According to this criteria, 55.8% of the clinical isolates population were similar to the strain of class IV
or homogenic resistant, 11.8% was found as I, and 32.3% were categorized as class II or III. However, continuous diversity
of measured features was also discussed. |
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