Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L. |
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| Authors: | C K Salunkhe P S Rao M Mhatre |
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| Institution: | (1) L and CM Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India, IN;(2) Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India Fax: +091-22-5505151; 5519613 e-mail: biotech@magnum.barc.ernet.in, IN |
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| Abstract: | Anthers of Vitis latifolia L. (wild grape) cultured on Nitsch and Nitsch medium supplemented with 20 μM 2,4-D and 9 μM BAP produced callus after 4–6 weeks. Subculture of callus onto Nitsch and Nitsch medium containing 10 μM NAA produced somatic embryos within 6 weeks. On growth regulator-free Nitsch and Nitsch basal medium somatic embryos converted
to plantlets in 6–8 weeks. One gram of callus produced more than 400 somatic embryos with 13.7% being converted to complete
plantlets, which were subsequently established in soil. Regenerated plants were found to have mixoploid populations of cells,
2n = 38 and n = 19.
Received: 23 May 1998 / Revision received: 21 September 1998 / Accepted: 10 October 1998 |
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| Keywords: | Vitis latifolia Anthers Somatic embryos Plantlets |
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