利用基因组数据分析水稻基因顺式作用元件

为了研究籼粳亚种基因调控序列的总体特性,我们利用籼粳稻以及拟南芥基因组和全长mRNA序列获取了大量高可信度的调控序列,通过这些序列,分析了水稻基因调控序列顺式作用元件(信号)的数量、分布以及与GC含量的关系等．研究结果表明:一些信号在水稻基因调控序列中发生显著的数量变化,同时一些信号数量在水稻与拟南芥基因间存在明显差异, 这说明这两种单双子叶植物间信号的使用上存在偏好,同时水稻不同类型基因以及特有与非特有基因间在信号的使用上也存在差异．这些差异信号的分布直接导致了调控序列GC含量的波动．本研究没有发现水稻籼粳两个亚种间在调控序列方面(顺式调节因子和GC含量等)存在明显差异．

Analysis of Rice Cis-Acting Elements Based on Genomic Sequences

In order to characterize regulatory region of oryza sativa ssp. indica and japonica in genome-scale, we mapped and collected near 600 regulatory sequences which were further used for analysis of rice cis-acting element(signal)'s amount, distribution and GC content, based on rice and arabidopsis genome and their full-length mRNA sequences under quality control. The results indicate that there are significant quantitative changes of cis-acting elements in rice regulatory region. A diverse signal usage between rice and arabidopsis are observed. Meanwhile the difference between different types of rice genes or rice specific and un-specific genes in signal usage are also recognized. Those different usages of signals result in fluctuation of GC content in regulatory region of genes. No significant difference in usage of cis-acting elements and GC content between two rice subspecies (indica and japonica) is found in this study.