Cytochalasin B inhibits phosphoinositide hydrolysis in rat hippocampal slices |
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Authors: | Jun-ichi Kitanaka Sadaaki Maeda Akemichi Baba |
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Institution: | (1) Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, 565 Suita, Osaka, Japan;(2) Department of Pharmacology, Faculty of Dentistry, Osaka University, 1-6 Yamadaoka, 565 Suita, Osaka, Japan |
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Abstract: | The effect of cytochalasin B on phosphoinositide (PI) hydrolysis was examined in rat hippocampal slices. Pretreatment of the slices with cytochalasin B caused a significant decrease in PI hydrolysis elicited by carbachol, norepinephrine, or by high K+. This effect was cytochalasin B dose- and time-dependent and was not mimicked by cytochalasin D, vinblastine, colchicine, or phloretin. In contrast, in 3H]inositol-prelabeled hippocampal membranes, cytochalasin B did not affect PI hydrolysis elicited by GTPS and GTPS plus carbachol. Similar result was obtained using the membranes prepared from the slices pretreated with cytochalasin B. The inhibitory effect of cytochalasin B on the carbachol-response was observed in SK-N-SH human neuroblastoma cells, but not in cultured rat astrocytes. These results indicate that cytochalasin B inhibits PI hydrolysis in neuron-specific manner and that its action may be an indirect cellular mechanism other than interaction with cytoskeleton elements. |
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Keywords: | Cytochalasin B phosphoinositide hydrolysis cytoskeleton neuronal cell hippocampus |
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